European Journal of Anatomy

Official Journal of The Spanish Society of Anatomy
Cover Volume 23 - Number 5
Eur J Anat, 23 (5): 325-332 (2019)

Liver ischemia/reperfusion injury, a setting in which the functional mass is reduced and the role of PDE5 inhibitor

Hesham N. Mustafa1, Gehan A. Hegazy2,3, Sally A. El Awdan4, Aliaa Amr Alamoudi2

1Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, KSA, 2Clinical Biochemistry Depart-ment, Faculty of Medicine, King Abdulaziz University, Jeddah, KSA, 3Medical Biochemistry Department, National Re-search Centre, Cairo, Egypt, 4Pharmacology Department, National Research Centre, Cairo, Egypt

ABSTRACT Liver ischemia reperfusion is induced during sur-gical procedures like liver transplantation and re-section. Multiple mechanisms have been postulat-ed to liver damage following liver ischemia reperfu-sion injury, such as oxidative stress and inflamma-tory reactions. The present study declares the pos-sible mechanism of tadalafil, toward modulating the inflammatory response. Forty-eight rats were divided into 4 groups as follows; Sham group sub-jected to midline laparotomy only. Tadalafil group administered Tadalafil 10 mg/kg intraperitoneal 45 min before sham operation. I/R (Ischemia-reperfusion) group, rats undergo 60 min of hepatic ischemia followed by 60 min of reperfusion. Tada-lafil + I/R group rats undergo a similar pattern of I/R after the treatment with Tadalafil 10 mg/kg, 45 min before ischemia. At the end of the reperfusion, the blood samples were collected for estimation of biochemical markers including liver enzymes using colorimetric assay method and serum: TNF-α (tumor necrosis factor-α), IL-6 (interleukin 6) le-vels, ICAM- 1 (Intercellular Adhesion Molecule-1) were measured. Tissues were evaluated by semi-quantitative and morphometrical approaches. Ta-dalafil succeeded in restoring normal levels of liverenzymes and ameliorating the oxidative stress as evidenced by decreasing MDA and restoring redu-ced glutathione levels in liver tissue homogenate. Also, Tadalafil exhibits anti-inflammatory effects, as it significantly decreased the levels of TNF-α, IL6 and ICAM-1. The findings are supported by BCL-2, TNF-α immunomarkers. It is concluded that modulation of the inflammatory response might be one of the mechanisms of Tadalafil-mediated he-patoprotection, so it is recommended as an adju-vant therapy in liver surgery.

Keywords: Ischemia/reperfusion injury ?? Oxidati-ve stress ?? Apoptosis ?? TNF-α ?? BCL-2

European Journal of anatomy
ISSN 2340-311X (Online)