Eur J Anat, 2 (2): 101-108 (1998)
Inverse effects of estradiol and testosterone on the vitro proliferation rate of rat VIP-immunoreactive pituitary cells
J. Carretero1,2, R.J. Vázquez2, M. Rubio2, M. Santos2, G. Vázquez2, F. Sánchez1,2, E. Blanco1,2, A. Martín-Clavijo2 and R. Vázquez1,2
1.-Laboratory of Neuroendocrinology, Institute of Neuroscience of Castilla y León. Faculty of Medicine, University of Salamanca. Spain2.-Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca. Spain
ABSTRACT Using double immunocytochemicallabelling for Proliferating Cell Nuclear Antigen(PCNA)and Vasoactive Intestinal Peptíde (VIP), a stucly was conducted to el ucidate the repercussions of various closes (ranging from 10-8 to 10-5M) of estracliol 01'testosterone (fram 1 to 24 hours) aclministered to monolayer pituitary cultures on the proliferation rate, expressecl as the PCNA labelling inclex, of VIP-immunoreactive ce lis(PCNA-LO.The results are comparecl with those obtainee! in control cultures. Estracliol inclucecl significant increases in the percentages of PC A- and VIP-immunoreactive cells at each clase assayed as early on as one hour postadrni nistration. The most efficient effect was obser ved for 1O-5Mestradiol. For all time-points assa yecl, the percentages of PCNA- and VIP-immunoreactive cells were hígher than in control dishes. Similar findings were observed when percentages of VIP-immunoreactive cells were analyzed. Testosterone decreasecl the per centages of VIP-immunoreactive or PCNA- and VIP-immunoreactive cells with respect to control dishes at all doses and time points analyzed; frorn 6 to 24 hours of treatment, the effects were less evident for 10-7 ane! 1O-8M testosterone than for the other doses assayed. The rnodifications observed in the proliferation rate ancl nurnerical density of VIP-immunoreactive cells were accornpanied by increases, in the case of estra diol, and decreases, in the case of testosterone,in the release of VIP to the culture médium. In conclusion, our results suggest that estradiol and testosterone have opposite effects on the relea se of VIP from pituitary monolayer cultures and on the regulation of the in uitro proliferation of pituitary VIP-immunoreactive cells.
Keywords: Pituitary - VIP - Gonadal steroids - Cellular proliferation - Irnmunocytochemistry
European Journal of anatomy
ISSN 2340-311X (Online)