Eur J Anat, 23 (6): 415-424 (2019)

Green tea extract protects the renal cortex against bisphenol A-induced nephrotoxicity in the adult male albino rat: a histological andimmunohistochemical study

Walaa A. Fadda¹, Asmaa S. Essawy^1,2, Manar A. Faried¹

¹Human Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Menoufia, Egypt,²Anatomy Department, IbnSina National College for medical Studies, Jeddah, Saudi Arabia

ABSTRACT Bisphenol A is a chemical used in the production of the plastic lining of food and beverage contain-ers. As plastics are used extensively in modern life, bisphenol A is liberated into the surrounding environment. The goal of this study was to illus-trate the histopathological effects of bisphenol A on the renal cortex with referral to the possible ameliorative effect of green tea extract and to throw more light on some underlying mechanisms, for the first time up to our knowledge, by which green tea extract exerted its effects against bi-sphenol A-induced nephrotoxicity. Forty adult male Sprague-Dawley rats were classified into four groups: Group I (control group); Group II (bisphenol A-treated group), received bisphenol by gavage 125 μg/kg once daily for 35 days; Group III (bisphenol A and green tea extract treated group), received bisphenol by gavage 125 μg/kg simulta-neously with 200 mg/kg/day green tea extract once daily for 35 days; and Group IV (green tea extract treated group), received 200 mg/kg/day green tea extract for 35 days by gavage. At the end of the study, rats were anesthetized and the kidney from all groups were extracted and examined histologi-cally and immunohistochemically. Deterioration of kidney structure was greatest in group II as compa-red to control group. Some of the renal corpuscles showed widening of the Bowmanâ??s capsule, shrun-ken degenerated glomerular tuft and dilated con-gested glomerular capillaries. Interstitial and intra-tubular hemorrhage was also observed. Moreover, there was a significant increase in the collagen deposition in bisphenol A-treated group in addition to up-regulation of inducible nitric oxide synthase (iNOS), Fas Ligand (Fas L), alpha smooth muscle actin (α-SMA) and desmin immunoreaction. The co-administration of green tea extract greatly reduced these nephrotoxic effects of bisphenol A exposure through its antioxidant,

Keywords: Kidney â?? Bisphenol â?? Oxidative stress â?? Fibrosis â?? Apoptosis â?? Green tea extract