TY  - JOUR
A1  - ,  
T1  - Myogenic differentiation induced by actinomycin D in human rhabdomyosarcoma cells: modification in the expression of MyoD and Id
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 1997
VL  - 1
SP  - 31
EP  - 36
UR  - http://www.eurjanat.com/web/paper.php?id=96010044
KW  - Rhabdomyosarcoma
KW  - cell differentiation
KW  - actinomycin D
KW  - creatine kinase
N2  - Neoplastic transformation may be an inability to respond adequarely to proliferation and differentiation signals. Studies in vivo showed that rhabdomyosarcomas, tumors originate de novo from the skeletal muscle, can acquire a higher degree of myogenic differentiation after cytotoxic treatment. We used the rhabdomyosarcoma cell line RD induced to differentiate with actinomycin D to analyze mmodifications in the degree of myogenic differentiation. Our results showed that a decrease in Id, a negative regulator of MyoD activity, may induce activation of muscle creatine kinase expression, which leads to a process of biochemical differentiation in these cells. Our findings suggest that the capacity of rhabdomyosarcoma cells to differentiate is not lost, and the necessary structural genes may still be operative. Consequently, differentition therapy with antineoplastic drugs may be used in rhabdomyosarcomas as a clinical alternative in the treatment of these tumors.
ER  -