TY  - JOUR
A1  - J, Carretero 
A1  - R, Hernandez 
A1  - M, Tapia 
A1  - D, Prieto 
A1  - M, Carretero 
A1  - S, Gutierrez 
A1  - R, Gonzalez 
A1  - M, Basco 
A1  - L, Gonzalez 
A1  - J, Herrero J.
A1  - M, Rubio 
A1  - E, Blanco 
A1  - R, Vazquez 
T1  - Immunocytochemical evidence for the coexistence of aromatase P450 and estrogen receptor ? in the pituitary gland of the adult male rat
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2009
VL  - 13
SP  - 1
EP  - 6
UR  - http://www.eurjanat.com/web/paper.php?id=09010001
KW  - aromatase
KW  - estrogen receptor alpha
KW  - animal cell
KW  - animal tissue
KW  - article
KW  - controlled study
KW  - enzyme activity
KW  - enzyme synthesis
KW  - hypophysis
KW  - hypophysis cell
KW  - immunocytochemistry
KW  - in situ hybridization
KW  - male
KW  - nonhuman
KW  - rat
KW  - reverse transcription polymerase chain reaction
N2  - In previous studies we demonstrated the immunocytochemical expression of aromatase in rat pituitary cells. The aim of the present study is to demonstrate the synthesis of the enzyme through the expression of the mRNA of the enzyme in pituitary cells by in situ hybridization and the relationship between the immunocytochemical expression of aromatase and estrogen receptor ? in adult male rats. In situ hybridization revealed that aromatase-mRNA is located in the cytoplasm of 41% of pituitary cells. By immunocytochemistry, different patterns of reaction for aromatase were found. Moreover, using immunocytochemistry and RT-PCR, all aromatase-positive tumors proved be estrogen receptor ?-positive tumors. The immunocytochemical coexistence of aromatase and estrogen receptor ? was found. These observations demonstrate the synthesis of aromatase in the pituitary gland and its functional ability to develop its estrogenic effects mediated by estrogen receptor ?.
ER  -