TY  - JOUR
A1  - ,  
T1  - Increases in the size, number and proliferation rate of VIP-immunoreactive pituitary cells induced in vitro by TRH are associated with increases in VIP release
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2003
VL  - 7
SP  - 91
EP  - 95
UR  - http://www.eurjanat.com/web/paper.php?id=03020091
KW  - cycline
KW  - protirelin
KW  - vasoactive intestinal polypeptide
KW  - animal cell
KW  - article
KW  - cell count
KW  - cell density
KW  - cell proliferation
KW  - cell size
KW  - hormonal regulation
KW  - hormone release
KW  - hypophysis cell
KW  - hypothyroidism
KW  - immunocytochemistry
KW  - male
KW  - neurosecretory cell
KW  - nonhuman
KW  - protein expression
KW  - rat
N2  - Pituitary VIP levels and pituitary VIP-expressing cell numbers are increased in hypothyroidism, but the regulating role of TRH as regards morphological changes remains obscure. In order to determine whether TRH is involved in regulating the maintenance of pituitary VIP-expressing cells and its cellular proliferation a double immunocytochemical study for VIP and proliferating cell nuclear antigen (PCNA) was carried out in pituitary monolayer cultures treated with TRH. TRH induced a significant in vitro increase in the numerical density (p<0.01) and percentage (p<0.001) of VIP-expressing cells. These changes were accompanied by increases in VIP release (p<0.01) and in the size (p<0.01) of VIP-expressing cells. Our results suggest that TRH could regulate the maintenance of the percentage of pituitary VIP-expressing cells, their activity and their proliferation in a similar way to the way in which it regulates the release of VIP.
ER  -