TY  - JOUR
A1  - ,  
T1  - Localization of the apoptosis repressor ARC and anti-apoptotic factor FLIP in endomyocardial biopsies of cardiomyopathy patients
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2002
VL  - 6
SP  - 161
EP  - 165
UR  - http://www.eurjanat.com/web/paper.php?id=02030161
KW  - ARC protein
KW  - caspase 8
KW  - DNA
KW  - FLICE inhibitory protein
KW  - repressor protein
KW  - unclassified drug
KW  - apoptosis
KW  - article
KW  - cardiomyopathy
KW  - enzyme localization
KW  - heart muscle biopsy
KW  - heart muscle contractility
KW  - human
KW  - human tissue
KW  - immunohistochemistry
KW  - protein analysis
KW  - protein expression
N2  - Endomyocardial biopsies from patients with dilated cardiomyopathy were obtained by cardiac catheterization. Tissue samples were embedded in epoxy resin and sectioned into serial semithin sections for immunohistochemical visualization of either the apoptosis repressor protein or the FLICE inhibitory protein. Colocalization of apoptosis repressor protein and FLICE inhibitory protein was observed in cardiac myocytes, with nuclear DNA fragmentation and myofibrillary degradation. Intact myocytes were devoid of FLICE inhibitory protein and apoptosis repressor protein staining. FLICE (Fas-associated death-domain-like IL-1-converting enzyme) inhibitory protein and apoptosis repressor protein were localized in the perinuclear cytoplasm, mainly in areas that showed myofibrillary degradation as visualized by fluorescence microscopy. FLICE inhibitory and apoptosis repressor proteins may be intracellular markers for monitoring myocardial damage in several cardiac diseases in humans.
ER  -