TY  - JOUR
A1  - ,  
T1  - The influence of carbon dioxide concentration on the neurite outgrowth of mouse embryonic cortical neurons in vitro
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2002
VL  - 6
SP  - 9
EP  - 11
UR  - http://www.eurjanat.com/web/paper.php?id=02010009
KW  - carbon dioxide
KW  - microtubule associated protein 2
KW  - animal tissue
KW  - article
KW  - brain
KW  - brain cell
KW  - cell density
KW  - cell structure
KW  - cell survival
KW  - cell viability
KW  - cellular distribution
KW  - concentration response
KW  - culture medium
KW  - embryo
KW  - immunocytochemistry
KW  - morphology
KW  - mouse
KW  - nerve cell culture
KW  - nerve fiber growth
KW  - nonhuman
KW  - synaptogenesis
N2  - The carbon dioxide (CO<sub>2</sub>) concentration of the incubation medium in nerve cell culturing has always been a matter of debate and is usually adjusted in a somewhat empirical manner. In the many laboratory protocols and manuals published to date, the data concerning this aspect differ. The goal of this study was thus to follow up the neurite outgrowth of cortical neurons in dissociated primary cultures obtained from seventeen-day-old embryonic mouse brains incubated in 90% air and 10% CO<sub>2</sub>, and in 95% air and 5% CO<sub>2</sub> respectively. We recorded the density and confluence of the neuronal distribution in vitro on the second, third and fifth day after establishing the cultures. Morphological evaluation to demonstrate viable cells was carried out by applying immunocytochemistry for microtubule-associated protein-2 due to its ubiquity in neurons. The results showed that neuronal survival, the rate of neurite outgrowth and the establishment of intercellular networks and synaptic contacts were more pronounced in cortical cultures raised in 10% CO<sub>2</sub>-containing incubation medium than in 5% CO<sub>2</sub>-containing incubation medium. From these results, it can be inferred that CO<sub>2</sub> at a higher concentration plays a significant role in influencing the metabolic activities of embryonic cortical neurons in vitro, thus probably determining their synaptogenesis and viability.
ER  -