European Journal of Anatomy

Official Journal of The Spanish Society of Anatomy
Cover Volume 24 - Number 3
Eur J Anat, 24 (3): 193-203 (2020)

Effect of methanol seed extract of Buchholzia coriacea on the leftventricle myocardium ofzidovudine-induced cardiotoxic Wistar rats

Josiah T. Mosaku1, Aderibigbe O. Komolafe1, Rotimi A. Owolabi2, Ayodeji S. Odukoya1, Stephen O. Saka1, Atinuke O. Anjorin1

1Department of Anatomy and Cell Biology, 2Department of Medical Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, Obafemi Awolowo University (OAU), Ile Ife, Osun-State, Nigeria

ABSTRACT The degenerative and inflammatory changes were reported in cardiac tissues of rats exposed to zidovudine (ZDV). This study was designed to ex-amine the histochemical changes in the myocardi-um of adult Wistar rats exposed to ZDV and ad-ministered with methanolic extract of Buchholzia coriacea (MEBC) seed. Forty-eight healthy Wistar rats weighing 150-155 g. were randomly assigned into eight groups of six rats each. Group A served as control and received distilled water; group B received 100 mg/kg of ZDV; group C received 600 mg/kg of MEBC; group D received 100 mg/kg of vitamin C; group E received 100 mg/kg of vitamin C and ZDV; group F received 150 mg/kg of MEBC and 100 mg/kg of ZDV; group G received 300 mg/kg of MEBC and 100 mg/kg of ZDV, and group H received 600 mg/kg of MEBC and 100 mg/kg of ZDV. Treatment lasted for a period of 56 days. Blood was collected separately into clean capped plain tubes for biochemical parameters. Heartswere excised, fixed in 10% formal saline and pro-cessed for histology. ZDV induced a significant increase in the serum concentration of Nitric Oxide (NO) and Cardiac Troponin I (cTnI) in the ZDV-alone group when compared to control (p<0.05). Also, there was reduction in activity of the Glutathi-one reductase (GR) enzyme in the ZDV-alone group relative to control (P=0.0006, F=7.0). Distor-tion of the cross banding pattern of cardiac muscle fibres in ZDV-alone group was manifested. These effects were reversed by administration of MEBC compared to vitamin C group.

Keywords: Buchholzia coriacea Zidovudine Heart Cardiotoxic

European Journal of anatomy
ISSN 2340-311X (Online)